Session IV: Cryopreservation and Other Preservation Methods for Rodent Models in Biomedical Research
1:00 p.m. – 5:00 p.m. EDT
October 16, 2024
Meeting Resources
Session IV Agenda
Session IV Objectives
Mice and rats are the most commonly used rodent species in biomedical research to uncover the genetic origins of human diseases. This session will focus on mice and rats’ germplasm cryopreservation, other long-term preservation, alternative transportation, practical rederivation methods, and potential genomic and epigenomic alterations because of cryopreservation and rederivation-induced interventions. This session will also discuss current approaches and challenges in preserving and rederivation of rodent models from repositories and rodent genome editing core facilities' perspective.
Agenda
| 1:00 p.m. – 1:10 p.m. |
Opening Remarks
Oleg Mirochnitchenko, Ph.D., Office of Research Infrastructure Programs, National Institutes of Health
Yuksel Agca, D.V.M., Ph.D., University of Missouri
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Presentations: Part 1
Moderator: Yuksel Agca, D.V.M., Ph.D., M.S., University of Missouri
| 1:10 p.m. – 1:35 p.m. |
Robert Taft, Ph.D., The Jackson Laboratory
Challenges and Opportunities Associated with Rodent Germplasm Cryobanking
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| 1:35 p.m. – 2:00 p.m. |
Toru Takeo, Ph.D., Kumamoto University
Cryopreservation of Rodent Sperm: Efficiencies, Reproducibility, and Needs
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| 2:00 p.m. – 2:05 p.m. |
Break
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Presentations: Part 2
Moderator: Thomas Saunders, Ph.D., University of Michigan
| 2:05 p.m. – 2:25 p.m. |
Yuksel Agca, D.V.M., Ph.D., M.S., University of Missouri
Cryopreservation of Rodent Oocytes: Efficiencies, Reproducibility, and Needs
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| 2:25 p.m. – 2:45 p.m. |
Simon Tröder, Ph.D., University of Cologne
SEcuRe: Adapting Proven Approaches for Affordable and Simplified Mouse In Vitro Fertilization
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| 2:45 p.m. – 3:05 p.m. |
Willem Wolkers, Ph.D., University of Veterinary Medicine Hannover
High-Temperature Storage of Rodent Sperm
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| 3:05 p.m. – 3:10 p.m.. |
Break
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Presentations: Part 3
Moderator: Robert Taft, Ph.D., The Jackson Laboratory
| 3:10 p.m. – 3:30 p.m. |
Ali Eroglu, D.V.M., Ph.D., Augusta University
Cryopreservation-Induced Epigenetic Alterations: A Neglected Aspect
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| 3:30 p.m. – 3:50 p.m. |
Marcello Raspa, Ph.D., European Mouse Mutant Archive
Distribution of Cryopreserved Mouse Spermatozoa in Dry Ice: A Multicentric Study
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| 3:50 p.m. – 4:10 p.m. |
Thomas Saunders, Ph.D., University of Michigan
Rodent Germplasm Cryopreservation and Assisted Reproduction: From a Transgenic Rodent Model Core Facility Perspective
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| 4:10 p.m. – 4:15 p.m. |
Break
|
| 4:15 p.m. – 5:00 p.m. |
Group Discussion
Moderator: Yuksel Agca, D.V.M., Ph.D., M.S., University of Missouri
Panelists: All committee members and speakers
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| 5:00 p.m. |
Closing Remarks
Oleg Mirochnitchenko, Ph.D., Office of Research Infrastructure Programs, NIH
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Organization Committee for Session IV
Yuksel Agca D.V.M., M.Sc., Ph.D., University of Missouri, agcay@missouri.edu
Rob Taft, Ph.D., Jackson Laboratory, rob.taft@jax.org
Ali Eroglu, D.V.M., Ph.D., Augusta University, aeroglu@augusta.edu
Oleg Mirochnitchenko, Ph.D., ORIP, NIH, oleg.mirochnitchenko@nih.gov
Monika Aggarwal, Ph.D., ORIP, NIH, monika.aggarwal@nih.gov
List of Panel Participants