Please note, all times are in Eastern Time.

Welcome to Day 1
9:45 a.m. – 9:55 a.m.	Welcome and Day 1 Introduction Terry Watnick, M.D., Professor of Medicine, Joan B. and John H. Sadler Professor in Nephrology, and Principal Investigator, PKD RRC Coordinating Site, University of Maryland School of Medicine
Session 1: Cutting-Edge Techniques or Models That Could Be Utilized for PKD Research
	Session Chairs: Stefan Somlo, M.D., Yale University School of Medicine Luis Menezes, M.D., National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
9:55 a.m. – 10:15 a.m.	Merging Novel Approaches in Genome and Phenome Science for PKD Research Nancy Cox, Ph.D., Professor, Vanderbilt University Medical Center
10:15 a.m. – 10:20 a.m.	Questions
10:20 a.m. – 10:40 a.m.	Vascularized Kidney Organoids: Integrating Stem Cell Differentiation with Bioprinting to Create Improved PKD Models Jennifer Lewis, Sc.D., Hansjörg Wyss Professor, Harvard John A. Paulson School of Engineering and Applied Sciences and Wyss Institute
10:40 a.m. – 10:45 a.m.	Questions
10:45 a.m. – 11:05 a.m.	A Human Kidney Organoid Platform for Autosomal Dominant PKD (ADPKD) Andy McMahon, Ph.D., Professor, Keck School of Medicine of University of Southern California
11:05 a.m. – 11:10 a.m.	Questions
11:10 a.m. – 11:30 a.m.	Cell Atlases as Roadmaps to Understanding Disease Orit Rozenblatt-Rosen, Ph.D., Genentech
11:30 a.m. – 11:35 a.m.	Questions
11:35 a.m. – 12:15 p.m	Trainee Short Communications
	(5 trainee abstracts, 5-minute presentations with 3 minutes for questions each, 1–3 slides) Session Chairs: Bernie Gitomer, Ph.D., University of Colorado Denver Takamitsu Saigusa, M.D., The University of Alabama at Birmingham
12:15 p.m. – 12:45 p.m.	Lunch Break
Challenge Sessions A and B—Concurrent
12:45 p.m. – 1:45 p.m.	Challenge A: Developing Biomarkers for Measuring PKD Disease Progression—Do We Know as Much as We Think We Do? Session Chairs: Ron Perrone, M.D., Tufts Medical Center Erum Hartung, M.D., MTR, Children’s Hospital of Pennsylvania
12:45 p.m. – 1:05 p.m.	Urinary Biomarkers in PKD: Potential Value and Link to Pathophysiology Harald Mischak, Ph.D., Mosaiques Diagnostics and Professor, University of Glasgow
1:05 p.m. – 1:25 p.m.	The Long Road to Biomarkers of Polycystic Kidney Disease Jon Klein, M.D., Ph.D., Professor, University of Louisville School of Medicine
1:25 p.m. – 1:45 p.m.	Panel Discussion: Ron Perrone, Erum Hartung, Neera Dahl, Jon Klein, Ronak Lakhia, Max Liebau, Harald Mischak, and Kristen Nowak
12:45 p.m. – 1:45 p.m.	Challenge B: The complexity of ADPKD pathogenesis in humans has made difficult the discovery of the intrinsic function of the polycystin protein complex. Current work supports a number of different signaling modalities for the polycystin complex, including functioning as an ion channel, a G protein-coupled receptor, and others. What tools are necessary to clarify how the polycystins function in signaling pathways? Session Chairs: Vishal Patel, M.D., Associate Professor, The University of Texas Southwestern Medical Center Rebecca Walker, D.Phil., University of Maryland School of Medicine
12:45 p.m. – 1:05 p.m.	Defining How the Polycystins Prevent Cyst Formation: Are We Ready to Fill the Gap? Alessandra Boletta, Ph.D., Group Leader, Ospedale San Raffaele
1:05 p.m. – 1:25 p.m.	Whom Do the Polycystins Talk to, What Do They Say, and How Can We Listen in? Michael Caplan, M.D., Ph.D., Professor, Yale University School of Medicine
1:25 p.m. – 1:45 p.m.	Panel Discussion: Vishal Patel, Rebecca Walker, Alessandra Boletta, Michael Caplan, Marcus Delling, Ken Hallows, Feng Qian, and Kurt Zimmerman
1:45 p.m. – 2:00 p.m.	Break
Controversy Sessions A and B—Concurrent
2:00 p.m. – 3:00 p.m.	Controversy A: Both haploinsufficiency and secondary somatic mutations of the PKD genes have been reported clinically. Is the frequency of one dominant? Which should the field focus on for model and therapeutic development? Session Chairs: York Pei, M.D., Professor, University of Toronto Cynthia Sieben, Ph.D., Mayo Clinic
2:00 p.m. – 2:20 p.m.	Insufficient Polycystin Function Is the Principal Cause of ADPKD–Does It Matter How Many “Hits” Cause It? Greg Germino, M.D., Deputy Director, Senior Investigator, NIDDK
2:20 p.m. – 2:40 p.m.	The Case for a Dosage/Threshold Model of Cyst Initiation in ADPKD Peter Harris, Ph.D., Professor, Mayo Clinic
2:40 p.m. – 3:00 p.m.	Panel Discussion: York Pei, Cynthia Sieben, Greg Germino, Peter Harris, Katharina Hopp, Matthew Lanktree, Hanna Rennert, and Chris Ward
2:00 p.m. – 3:00 p.m.	Controversy B: Is PKD Only a Ciliopathy? Is It Time to Reassess the Role of Polycystin Function Outside the Cilia in Human Disease? Session Chairs: Barbara Ehrlich, Ph.D., Professor, Yale University School of Medicine Eryn Dixon, Ph.D., Washington University School of Medicine in St. Louis
2:00 p.m. – 2:20 p.m.	PKD Is a Ciliopathy Greg Pazour, Ph.D., Professor, University of Massachusetts Medical School
2:20 p.m. – 2:40 p.m.	Evidence Supporting a Role of Polycystins Outside the Cilia Michael Köttgen, M.D., Professor, University of Freiburg
2:40 p.m. – 3:00 p.m.	Panel Discussion: Barbara Ehrlich, Eryn Dixon, Michael Köttgen, Karel Liem, Moe Mahjoub, Greg Pazour, and Brad Yoder
3:00 p.m.	Adjournment

Welcome to Day 2
10:00 a.m. – 10:05 a.m.	Day 2 Introduction Brad Yoder, Ph.D., Professor of Cell Biology, Endowed Chair in Biomedical Research
10:05 a.m. – 11:45 a.m.	Session 2: Biology and Function of the Polycystins
	Session Chairs: Dorien Peters, Ph.D., Professor, Leiden University Medical Center Whitney Besse, M.D., Assistant Professor, Yale University School of Medicine
10:05 a.m. – 10:25 a.m.	Renal Plasticity Revealed Through Reversal of Polycyctic Kidney Disease in Mice Ke Dong, M.D., Yale University School of Medicine
10:25 a.m. – 10:30 a.m.	Questions
10:30 a.m. – 10:50 a.m.	Potential Role of AMP-Activated Protein Kinase in Metabolic Reprogramming Greg Steinberg, Ph.D., Professor, McMaster University
10:50 a.m. – 10:55 a.m.	Questions
10:55 a.m. – 11:15 a.m.	How Cilia Decipher cAMP Jeremy Reiter, M.D., Ph.D., Professor, University of California, San Francisco
11:15 a.m. – 11:20 a.m.	Questions
11:20 a.m. – 11:40 a.m.	Understanding Polycystic Kidney Disease Through the Lens of the Electron Microscope Qiang Su, Ph.D., Westlake University
11:40 a.m. – 11:45 a.m.	Questions
11:45 a.m. – 12:15 p.m.	Lunch Break
12:15 p.m. – 1:15 pm	Trainee Short Communications
	(7 trainee abstracts, 5-minute presentations with 3 minutes for questions each, 1–3 slides) Session Chairs: Bernie Gitomer, Ph.D., Professor, University of Colorado Denver Takamitsu Saigusa, M.D., Associate Professor, The University of Alabama at Birmingham
1:15 p.m. – 2:30 p.m.	Challenges and Controversies Sessions Day 2
	Moderator: Stefan Somlo, M.D., Yale University School of Medicine All topic groups and panelists meet as one. Challenge Session A: Developing Biomarkers for Measuring PKD Progression Chairs: Ron Perrone and Erum Hartung Challenge Session B: The Complexity of ADPKD Pathogenesis in Humans Chairs: Vishal Patel and Rebecca Walker Controversy Session A: Haploinsufficiency vs. Secondary Somatic Mutations Chairs: York Pei and Cynthia Sieben Controversy Session B: Is PKD Only a Ciliopathy? Chairs: Barbara Ehrlich and Eryn Dixon Sessions chairs for each topic group will present the distillation of the previous day’s discussions with the support of the panelists from their respective groups. Each topic presentation will be followed by a moderated discussion with the entire conference audience.
2:30 p.m. – 3:00 p.m.	Community Feedback—Critical Needs
	Breakout sessions will be hosted by each of the consortium divisions and focused on feedback from the community on what their critical needs are for new models and tools. Breakout 1: In Vitro Models, Chair: Darren Wallace Moderators: Lisa Guay-Woodford and Alan Yu Breakout 2: Animal Models, Chair: Brad Yoder Moderators: Patricia Outeda-Garcia and Pamela Tran Breakout 3: Clinical Resources, Chair: Steve Seliger Moderators: John Parant and Owen Woodward
3:00 p.m.	Adjournment Thank you for attending our First Annual Virtual PKD-RRC Symposium!